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Reproductive diapause in males of the linden bug, Pyrrhocoris apterus
HEJNÍKOVÁ, Markéta
This thesis focuses on the male reproductive diapause in the linden bug, Pyrrhocoris apterus. We examined in detail the response of males to changing photoperiodic conditions, the change in levels of juvenile hormone, and its role in male diapause, as well as the effect of low temperature on diapause termination. We compared the physiology of males and females and pointed out important sexual dimorphism in the control of diapause. Finally, we analyzed a completely novel insect neuropeptide to and explored its possible link in the chain of control of reproductive diapause.
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Characterisation and regulation of muscarinic and adrenergic receptors Subtitle: The effect of stress on muscarinic and adrenergic receptors in the lung and in the heart
Nováková, Martina ; Mysliveček, Jaromír (advisor) ; Hynie, Sixtus (referee) ; Mravec, Boris (referee)
The aim of this thesis was to clarify the influence of the stress on the adrenergic and muscarinic receptors in the heart and in the lungs. Research was perform on rat hearts and lungs and on the hearts and lungs of the CRH KO mice. First, we assessed mRNA levels of all α- and β-adrenergic receptor and muscarinic receptor subtypes. Subsequently, we performed the radioligand-binding studies to determine densities of these receptors. We identified all three α1-adrenergic receptor subtypes in the rat lungs. In the lungs of WT mice, we found that the amount of α1-adrenergic and muscarinic receptors was sex-dependent. Densities of the former were higher in females and those of the latter were higher in males. There was no difference between males and females in β-adrenergic receptor density. As for CRH KO mice, the basal densities of studied receptors were lower than in CRH WT mice (except β1-adrenergic receptors in females). The main purpose of the thesis was to detect the immobilization-induced changes in the studied receptors in the kontrol (WT) and CRH KO mice. Short-term and long-term immobilization caused decrease in all α1-adrenergic receptor subtypes in females, whereas only α1A-adrenergic receptors decreased in males. The amount of β1-adrenergic receptors decreased in males and remained without...
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Characterisation and regulation of muscarinic and adrenergic receptors Subtitle: The effect of stress on muscarinic and adrenergic receptors in the lung and in the heart
Nováková, Martina ; Mysliveček, Jaromír (advisor) ; Hynie, Sixtus (referee) ; Mravec, Boris (referee)
The aim of this thesis was to clarify the influence of the stress on the adrenergic and muscarinic receptors in the heart and in the lungs. Research was perform on rat hearts and lungs and on the hearts and lungs of the CRH KO mice. First, we assessed mRNA levels of all α- and β-adrenergic receptor and muscarinic receptor subtypes. Subsequently, we performed the radioligand-binding studies to determine densities of these receptors. We identified all three α1-adrenergic receptor subtypes in the rat lungs. In the lungs of WT mice, we found that the amount of α1-adrenergic and muscarinic receptors was sex-dependent. Densities of the former were higher in females and those of the latter were higher in males. There was no difference between males and females in β-adrenergic receptor density. As for CRH KO mice, the basal densities of studied receptors were lower than in CRH WT mice (except β1-adrenergic receptors in females). The main purpose of the thesis was to detect the immobilization-induced changes in the studied receptors in the kontrol (WT) and CRH KO mice. Short-term and long-term immobilization caused decrease in all α1-adrenergic receptor subtypes in females, whereas only α1A-adrenergic receptors decreased in males. The amount of β1-adrenergic receptors decreased in males and remained without...
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Induction and optimalization out-of-season spawning of spawners pikeperch (Sander lucioperca)
VLČEK, Jakub
In total, 21 pairs of pikeperch brood stock were divided into three groups and used during two out-of-season and one seasonal semi-artificial spawning. The only differences among these three groups were the length of cold water period (CWP) and spawning date. Group of earlier spawning (A) CWP = 122 days, Group of normal spawning (B) CWP = 149 days, Group of late spawning (C) CWP = 223 days). Dates of spawnings A 11.3., B 26.4., C 13.6). There were observed and assessed the main production parameters such us latency, successful spawning, fertilisation and hatching rate etc. in each group and spermatozoa quality (sampled one day after the observed spawning). In Group A and B, 100 % of fish successfully spawned. Fertilisation rate was 59.4 % (Group A) and 80.3 % (Group B). Much worse results were obtained in Group C. Only 42.9 % of fish spawned and the fertilisation rate was zero. There was no difference among the three groups in spermatozoa quality parameters. It means that the poor fertilisation rate in Group C was caused by low egg quality. According to our results we can say that it is possible to successfully spawn pikeperch earlier (before the main spawning season) with comparable result to the natural spawning season. However, it is necessary to find some better way for female stimulation to provide higher egg quality and subsequently higher fertilisation rate.
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